Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 93)

7.9.2 Collecting Purified Sample
The purified sample is collected in fraction tube position 1D or 2D. When the purified
protein is collected, the Run in Progress screen displays the actual elution times and the tube
can be removed. After the target protein has eluted, the Profinia system will automatically
continue to run the cartridge cleaning and storage procedures and process the second sample
if selected.
7.9.3 Completing the Run
Run results display as soon as the end-of-run cleaning procedures are completed.
7.10 Preparing System for End-of-Run Cleaning
System cleaning is an automatic feature that occurs upon completion of each method run
and is required to prevent cross-contamination between runs and to maintain the system
for optimum operation. The two sample lines and the fraction line valve (all eight fraction
lines) will be cleaned as part of the end-of-run cleaning process. The pump seals are also
washed as the final step in this process and the internal system lines are cleaned with
20% ethanol from buffer position 8.
7.10.1 Preparing for Line Cleaning
To prepare the system for end-of-run cleaning:
1. Remove all sample tubes from the instrument.
2. Place tubes containing 10 ml of deionized water in both sample 1 and sample 2 positions;
place water in both sample positions even if only one sample was used in the run.
3. Fill a buffer bottle with 20% ethanol and place it in buffer position 8.
4. Remove all fraction collection tubes from the instrument.
5. Place the cleaning tray (provided with the instrument) in the fraction collection area.
6. Touch the Start button to begin the cleaning procedure.
You will proceed to the System Cleaning in Progress screen.
7.10.2 System Cleaning in Progress Screen
When system cleaning starts, the System Cleaning in Progress screen displays the four
system cleaning procedures: Clean Sample Lines, Clean Fraction Lines, Clean Buffer
Lines, and System EtOH Wash. The cleaning steps that will be performed after a run is
completed are indicated by darkened circles. A checkmark adjacent to a darkened circle
indicates the cleaning step has been completed. When the cleaning process is completed,
you will proceed to the Run Completed screen and the run results are displayed.
7.11 Performing End-of-Run Procedures
The Run Completed screen (Figure 7.16) is displayed on completion of all purification run
steps, including system cleaning. Run details for each sample purified are displayed in the
run data table. The total estimated protein (yield) and protein concentration have been
calculated and are displayed for each sample.
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