Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 18)

3. Place the tube in the sample 1 position and select the “1 Sample” option in the appropriate
run set-up screen.
4. Prepare the buffers and reagents as described in the starter kit instruction manual.
5. Follow the set-up steps in section 4 (quick guide) or in section 6 (Bio-Rad methods
programming) of this manual to select the method and load the buffers, sample,
cartridges, and fraction tubes.
6. Compare your results to the following expected results for the IMAC or GST purification
of the control lysate.
Expected Results for IMAC Purification of the Control Lysate
Fig. 2.9. Typical chromatogram of the native IMAC control lysate purification with desalting.
Fig. 2.10. Typical gel electrophoresis results of the native IMAC control lysate purification. Lane 1,
Precision Plus
™ MW standard; 2, load; 3, flow-through; 4, wash 1; 5, wash 2; 6, eluted protein.
11
Gel lanes
1 2 3 4 5 6
MW
250
150
100
75
50
37
25
20
15
10