Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 108)

Outlet select valve (OSV) — rotary valve (10 port) that directs system output. After a valve
position is entered in the Edit menu and you touch the Update button on the Manual
Operation screen, the valve moves
• Positions 1 through 4 are fractions 1A through 1D
• Positions 5 through 8 are fractions 2A through 2D
• Position 9 is the fraction collector outlet
• Position 10 is the system waste outlet
Column valve 1 (CV1) — rotary valve (6 port) that directs the flow for cartridge 1. After a
valve position is entered in the Edit menu and the Update button is pressed on the Manual
Operation screen, the valve will move
• Position 1 directs flow to the system waste outlet
• Position 2 directs flow to flush behind the pump pistons
• Position 5 directs flow to column valve 2
• Position 6 directs flow to cartridge 1
• All other valve ports are plugged
Column valve 2 (CV2) — rotary valve (6 port) that directs the flow for cartridge 1. After a
valve position is entered in the Edit menu and the Update button is pressed on the Manual
Operation screen, the valve will move
• Position 4 directs flow to the system waste outlet
• Position 5 directs flow to the conductivity monitor, pH monitor (optional) and to the
outlet select valve
• Position 6 directs flow to cartridge 2
• All other valve ports are plugged
Proportioning valve (PV) — solenoid valve (3 port, 2 position). The proportioning valve
mixes concentrated buffer from the inlet select valve with water from the system water
bottle. Position settings are 0x, and 1x through 5x:
• 0x draws 100% water from the water bottle
• 1x draws 100% buffer from the inlet select valve (ISV)
• 2x makes a 1:1 concentrate/water mixture
• 3x makes a 1:2 concentrate/water mixture
• 4x makes a 1:3 concentrate/water mixture
• 5x makes a 1:4 concentrate/water mixture
The proportioning valve operates only when the pump is running. The timing of the valve
depends on the pump flow rate
Warning: Use care when controlling components manually. Improper settings may damage
the cartridges or system components.
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