Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 47)

Fig. 6.10. Install Reagents, Sample & Fraction Tubes screen (left) with arrows demonstrating the correlation
between the diagram and placement of reagents in the Profinia instrument (right).
The installation screen diagram displays:
• Buffer bottles — eight buffer bottle positions indicated by labels B1 through B8 on the
touch screen (top of the screen) and 1 through 8 on the instrument (upper tray). The
touch-screen diagram also displays the minimum buffer volumes required for the selected
method; an “X” indicates no reagent is required at that location
Note: Profinia purification kit reagent bottles are labeled with the instrument buffer position
numbers, which are also displayed on the instrument Install Reagents, Sample & Fraction
Tubes screen.
• Waste bottle — left side of the touch-screen diagram and positioned at the lower left of
the instrument. On the touch screen, the waste bottle is displayed without a volume as
a reminder to make sure the waste bottle is emptied frequently
• Diluent (water) bottle — right side of the touch-screen diagram and positioned at the
lower right of the instrument. On the touch screen, the diagram is labeled with the
minimum volume of water required for the selected method run
• Sample tubes — indicated by S1 and S2 labels on the touch screen, the sample tubes
are placed beneath the buffer bottles toward the right of the instrument. The touch
screen displays one or two sample tubes with the sample volumes, depending on the
number of samples and sample volumes that have been entered by the user on the
Enter Run & Sample Information screen
Note: Each run primes the sample line(s) with about 1 ml of sample. To ensure that the
entered sample volume is loaded onto the cartridge, filling the sample tubes with 1 ml more
than the entered volume is recommended. If the exact entered volume is added to the
sample tube, the end of sample detection, if activated, applies the complete sample to the
system. In this case, the total amount of sample added to the cartridge will be approximately
0.5 ml less than the entered volume. To maximize protein yields, 10 ml is the minimum
recommended sample load.
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