Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 122)

5. Select the OK button to confirm selections and return to the Diag/Maint Functions
screen.
Fig. 9.18. Select End of Sample/Reagent Detection screen.
9.3.15 Selecting Method Temperature
For methods that include the desalting step, the instrument operating temperature is critical.
Maximum flow rates for the desalting cartridges are lower at 4°C than at room
temperature. Flow rates over maximum limits compress the bed volume and cause
over-pressure errors. Make sure the operating temperature is set to match cold room or
room temperature operating conditions before programming your run. Methods with desalting
steps have longer run times at the cold room setting due to the lower maximum flow rates.
The operating temperature setting displays as a status indicator in the upper right corner of
the screen. If the cold room operating temperature is selected, “C” appears only when
methods are selected that include a desalting step. If the room temperature setting is
selected, “C” will not display. For affinity-only methods, the temperature setting is irrelevant
and will not display. See Table 6.1 (section 6) for flow rate details. To set the operating
temperature:
1. Touch the Data/Utilities button on the system home screen.
2. Touch the Diag/Maint Functions button.
3. On the Diag/Maint Functions screen, use the up and down arrows to scroll through the
list of functions and highlight ”Select Method Temperature”, then touch the Select button.
The Select Method Temperature screen will display (Figure 9.18).
4. Use the up and down arrow buttons to select room temperature or cold room (4°C)
operation.
5. Touch the OK button to return to the Diag/Maint Function screen.
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