Bio-Rad Profinia™ Protein Purification Instrument User Manual
Page 217
210
Method Type:
Method Name:
eXact Cartridge Size:
# Cartridges:
Sample 1 Name & Volume:
Sample 2 Name & Volume:
Method Step
Step Description
Position/
Reagent
Buffer
Conc.,
1-5X
Frac
#
(S1/S2)
Flow
Rate
ml/min
CV
Peak
Detect
Delay
Max
Peak
Detect
Vol
Flow
Rate
ml/min
CV
Peak
Detect
Delay
Max
Peak
Detect
Vol
PRIMING STEPS
Not editable
x
w/w
x
x
x
x
x
x
x
x
EQUILIBRATING C1/C2
Equilibrate C1/C2 Equilibrate with
equilibration/wash-1 buffer
B1/equilibration
wash-1 buffer
w/w
x
x
x
x
LOADING SAMPLE
Load sample to C1 Load sample to eXact
cartridge C1
x
1A/2A
x
x
x
x
x
Load sample to C2 Load sample to eXact
cartridge C2
x
2A
x
x
x
WASHING
Wash-1 C1/C2 Wash eXact cartridge with
equilibration/wash-1 buffer
B1/equilibration
wash-1 buffer
1B/2B
x
x
x
x
Wash-2 C1/C2
(for 2-wash methods)
Wash eXact cartridge with
wash-2 buffer
B2/wash-2
buffer
1C/2C
x
x
INCUBATION-ELUTION
Prime elution buffer Not Editable
B3/elution buffer
x
w/w
x
x
x
x
x
x
x
x
Pre-elute C1-C2 Fill eXact cartridge with
elution buffer
B3/elution buffer
w/w
Incubation Enter 0 to 999 hrs.
x
__Hrs
x
x
x
__Hrs
x
x
Elute-1 C1/C2 Start elution until affinity
peak is detected
B3/elution buffer
w/w
x
x
Elute-2 C1C2 Collect affinity peak
B3/elution buffer
1D/2D
x
x
x
x
CARTRIDGE CLEAN
Clean-1 C1/C2 Clean eXact cartridge
B1/equilibration
wash-1buffer
w/w
x
x
x
x
Clean-2 C1/C2 Clean eXact cartridge
B6/regeneration
buffer
w/w
x
x
x
x
Clean-3 C1/C2 Clean eXact cartridge
B1/equilibration
wash-1 buffer
w/w
x
x
x
x
STORING
Store C1/C2 Store eXact cartridge
B7/storage solution
w/w
x
x
x
x
Peak Parameter
Settings
Peak Parameter
Settings
SAMPLE 1 SETTINGS
SAMPLE 2 SETTINGS
Sample 1&2
Settings
eXact Affinity only Program Method Planning Worksheet