Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 15)

2.2 First-Time Use of the Profinia System
Before using the Profinia system, perform buffer, sample, and fraction line cleaning
procedures to flush the system and remove any air trapped in the lines.
2.2.1 Preparing the Instrument for Cleaning
Fig. 2.6.a. Profinia system ports without bottles and tubes; 2.6.b. Profinia cleaning tray placement in the
fraction collection area.
Note: For detailed instructions on installing reagent bottles, sample tubes, fraction tubes,
and using cartridge connections, see section 6.4 and 6.5.
To prepare the Profinia system for cleaning prior to its first use:
1. Remove the plastic bag covering the Profinia instrument and remove the “No Lift” stickers
from the top cover.
2. Turn the instrument around so the front faces you, and turn the system on using the
switch on the lower right in front of the diluent (water) bottle.
3. Open the instrument top cover. Remove the plastic caps from the ends of the buffer
and sample tube lines.
8
a.
b.
Buffer tray area
Cartridge
holders
Waste
bottle
holder
Sample
loading area
Diluent (water)
bottle holder
Fraction collection area