Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 10)

The following accessories and components can be ordered separately for use with the
Profinia system:
• Profinia control lysate — dual-tag (histidine and GST) protein that can be used to
validate the performance of the Profinia system
• Profinia software — provides data reporting and basic analysis of run results
• Cooling accessory — holds two 15 ml or 50 ml conical tubes (holds two samples and/or
two purified protein fraction tubes), and is designed to keep samples or purified protein
cooled below 4°C when a cold room environment is not available
• Profinia purification kits — prepackaged cartridges, buffers, and reagents, for use
with Bio-Rad methods. Bottles and accessories are clearly labeled to indicate positioning
in the Profinia instrument (see Table 1.1)
• Profinia buffer kits — prepackaged buffer and reagents for use with Bio-Rad methods;
cartridges are not included. Bottles and accessories are clearly labeled to indicate
positioning in the Profinia instrument (see Table 1.1)
• Bio-Scale ™ Mini cartridges — IMAC, GST, Protein A cartridges (1 ml and 5 ml),
Profinity eXact cartridges (1 ml and 5 ml), and desalting cartridges (10 ml and 50 ml)
• Desalting sample loops — required for use in desalting methods only; available in 2 ml
and 10 ml sizes. This accessory is not required for Bio-Rad methods that include affinity
and desalting (for example, native IMAC and desalting or GST and desalting)
• Bottle starter pack — replacement empty buffer bottles (4 x 125 ml and 4 x 250 ml buffer
bottles, eight buffer lids)
• Waste/diluent bottle set — replacement waste and diluent bottles with caps and tubing
Table 1.1. Types of Profinia purification and buffer kits.
Method Purification Kit Buffer Kit
Native IMAC* 1 ml or 5 ml affinity cartridge Buffers and reagents for native
plus 10 ml or 50 ml desalting IMAC and desalting methods
cartridge configurations
Denaturing IMAC 1 ml or 5 ml affinity cartridge Buffers and reagents for
configurations; desalting does denaturing IMAC methods
not apply to this kit
GST** 1 ml or 5 ml affinity cartridge Buffers and reagents for GST
plus 10 ml or 50 ml desalting and desalting methods
cartridge configurations
Desalting 10 ml or 50 ml cartridge Buffers and reagents for
configurations desalting-only methods
* IMAC = immobilized metal affinity chromatography.
** GST = glutathione S-transferase.
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