Bio-Rad Profinia™ Protein Purification Instrument User Manual
Page 218
211
Method Type:
Method Name:
eXact Cartridge Size:
Desalting Cartridge Size:
Sample 1 Name & Volume:
Sample 2 Name & Volume:
Method Step
Step Description
Position/
Reagent
Buffer
Conc.,
1-5X
Frac
#
(S1/S2)
Flow
Rate
ml/min
CV
Peak
Detect
Delay
Max
Peak
Detect
Vol
Flow
Rate
ml/min
CV
Peak
Detect
Delay
PRIMING STEPS
Not Editable
x
w/w
x
x
x
x
x
x
x
EQUILIBRATING C2
Equilibrate Di-C2 Equilibrate DS Cartridge
with Di water
Di Water
x
w/w
x
x
x
Equilibrate C2 Equilibrate DS Cartridge
with desalting buffer
B4/Desalting
buffer
w/w
x
x
x
EQUILIBRATING C1
Equilibrate C1 Equilibrate with
Equilibration/Wash-1 buffer
B1/equilibration
Wash-1buffer
w/w
x
x
x
LOADING SAMPLE
Load Sample to C1 Load sample to eXact
cartridge C1
x
1A/2A
x
x
x
x
x
WASHING
Wash-1 C1/C2 Wash eXact cartridge with
Equilibration/Wash-1 buffer
B1/equilibration
Wash-1buffer
1B/2B
x
x
x
Wash-2 C1/C2
(for 2-Wash methods)
Wash eXact cartridge with
Wash-2 buffer
B2/Wash-2
buffer
1C/2C
x
x
x
INCUBATION-ELUTE to DS
Prime Elution buffer Not Editable
B3/Elution buffer
x
w/w
x
x
x
x
x
x
x
Pre-EluteC1 Fill eXact Cartridge with
Elution buffer
B3/Elution buffer
w/w
Incubation Enter 0 to 999 hrs.
x
__Hrs
x
x
x
__Hrs
x
Elute-1 C1 Start elution until affinity
peak is detected
B3/Elution buffer
w/w
x
x
Elute-2 C1 Divert affinity peak To DS
B3/Elution buffer
w/w
x
x
x
ELUTION DS
Elute-1 C2 Start elution until protein
peak is detected
B4/Desalt buffer
w/w
x
x
Elute-2 C2 Collect protein peak
B4/Desalt buffer
1D/2D
x
x
x
Elute-3 C2 Remove salts from DS
B4/Desalt buffer
w/w
x
x
x
CARTRIDGE CLEAN
Clean-1 C2 Clean Desalt Cartridge
B5/Clean-1 solution
w/w
x
x
x
Clean-2 C2 Clean Desalt Cartridge
B6/Regeneration
buffer w/w
x
x
x
Clean-1 C1 Clean eXact Cartridge
B1/equilibration
Wash-1buffer
w/w
x
x
x
Clean-2 C1 Clean eXact Cartridge
B6/Regeneration
buffer w/w
x
x
x
Clean-3 C1 Clean eXact Cartridge
B1/equilibration
Wash-1buffer
w/w
x
x
x
STORING
Store C1 Store eXact Cartridge
B7/Storage solution
w/w
x
x
x
Store C2 Store Desalt Cartridge
B7/Storage solution
w/w
x
x
x
SAMPLE 2 SETTING
Sample 1&2
Settings
Peak Parameter
Settings
Peak Pa
Setti
SAMPLE 1 SETTINGS
eXact Affinity + Desalting Program Method Planning Worksheet