Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 39)

To begin a Bio-Rad method purification procedure, touch the Bio-Rad Methods button in
the lower toolbar of the home screen (Figure 6.3). You will proceed to the Select Method
Type & Options screen.
Fig. 6.3. System home screen.
6.1 Selecting Method System
The Select Method system screen (Figure 6.4) allows you to choose:
• Method system — IMAC, GST, Protein A and G, Profinity eXact methods with or without
desalting, or desalting only methods
6.2 Selecting Method Type and Options
The Select Method Type & Options screen (Figure 6.5) allows you to choose:
• Method type — The selected affinity method system or desalting only system
• Number of samples — program one or two samples (the desalting-only method can
only be run with one sample)
• Cartridge size — based on sample volume and concentration
• Number of cartridges — available for affinity-only methods when two samples are
selected. You may choose to use a single cartridge for both samples or a separate
cartridge for each sample
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