Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 50)

Important: It is important to orient the buffer bottles properly in the instrument; the graduated
marks on the buffer bottles are used to determine how much buffer is available for the
purification run, so you must be able to see them (Figure 6.14).
7. Close the Profinia lid to protect the buffer bottles and sipper tubes during operation
(Figure 6.15).
Fig. 6.14. Proper placement of buffer bottles in the instrument, with orientation marks visible; buffer caps
are stored above respective bottle positions.
Fig. 6.15. Buffer bottles properly placed in all positions and lid closed in preparation for instrument
operation. The native IMAC plus desalting buffer setup is shown.
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Buffer
bottle cap
Buffer
sipper tube
Buffer
position
number
Buffer
bottle lid
Buffer
bottle
graduated
marks