Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 208)

Low purity of target protein Sample load and wash steps Select two wash option for eXact
on SDS-PAGE or residual do not return to baseline with method during setup to add
contamination from standard eXact method secondary wash step and/or
starting material wash option decrease load volume to
prevent over saturation of resin;
increase ionic strength of buffers
(see eXact System Manual for
details)
Section 4
Ordering Information
Catalog # Description
156-3004 Profinity eXact Antibody Reagent
750-0603 1/8” OD
PTFE
Tubing
731-8211 1.6 mm ID, 0.8 mm Wall Silicone Tubing
620-0401 Profinia Cooling Accessory
620-0404 Profinia Inline Filter Assembly Replacement
620-0228 Profinia Desalting Kit
620-0224 Profinia Desalting Buffer Kit , 10 applications
732-4646 BioScale Mini eXact Cartridges , 2 X 1 ml
732-4648 BioScale Mini eXact Cartridges , 1 X 5 ml
732-5312 BioScale Mini Profinity Desalting Cartridges , 1 X 50 ml
620-0216 5x Profinia Desalting Buffer , 200 ml
620-0217 2x Profinia Cleaning Solution ,1, 125 ml
201