Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 16)

4. Fill the four 125 ml and three of the 250 ml buffer bottles provided with the instrument to
capacity with deionized water, and place them in buffer positions 1 through 7.
5. Fill one buffer bottle with 100 ml of 20% ethanol and place it in buffer position 8.
6. Fill two 50 ml conical tubes with 25 ml of deionized water each, and place them in the
sample 1 and 2 (S1 and S2) positions.
Note: Tubes containing water must be placed in both sample positions during line cleaning,
even after performing a run with only one sample (line cleaning only).
7. Locate the cartridge holder fittings at the left center of the instrument. Secure the upper
cartridge holder fittings to the lower cartridge holder fittings by sliding the upper fittings
down until they contact the lower fittings. Tighten the black lock ring on each fitting by
turning it clockwise; do not overtighten (see section 6.5 for more details).
8. Place the cleaning tray in the fraction collection position (Figure 2.6).
9. Fill the diluent bottle with deionized water and place it in the right-side bottle position,
with the tube in place.
10. Make sure the waste bottle is empty and place it in the left-side bottle position, with the
tube in place.
Note: Make sure the waste bottle is empty before running any method or cleaning procedure.
2.2.2 Selecting the First-Time Cleaning Procedure
To flush the system and prepare it for first use:
1. Make sure the water and ethanol solutions are placed as described in steps 4 through 6
above, and the cleaning tray is in place, as described in step 8 above.
2. Touch the Data/Utilities button in the lower toolbar of the system home screen (see
section 5 for information on home screen functions).
3. Touch the arrow buttons to scroll through the list of functions and highlight “Clean All
Inlet and Outlet Lines” (Figure 2.7). Select Start to perform this procedure. A new
screen will display the progress toward completion. When the cleaning procedure is
complete, the system will return to the Data/Utilities screen.
Fig. 2.7. Clean all Inlet and Outlet Lines screen.
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