Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 73)

Fig. 6.42. Prepare System to Clean Lines, short-term wash selected screen.
To prepare the system for the short-term wash, follow the onscreen instructions:
1. Place bottles with deionized water in buffer positions 1 through 7.
2. Fill a buffer bottle with 20% ethanol and place it in buffer position 8.
3. Remove all cartridges and directly connect the upper and lower cartridge fittings.
4. Place the Profinia cleaning tray in the fraction collection port.
5. Touch the Start button to begin short-term storage cleaning; the cleaning progress
screen displays. Immediately below the progress bar, the current line cleaning is indicated.
When the cleaning process is completed, you will automatically return to the home screen.
Cleaning the System for Long-Term Storage
If the Profinia system will not be used within the next 2 days, it should be rinsed with water
and 20% ethanol or other sanitizer to maintain optimum mechanical performance and
prevent bacterial growth. To perform a long-term storage wash, touch the Long-Term Wash
button in the toolbar. You will proceed to the Prepare System to Clean Lines, long-term wash
screen (Figure 6.43).
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