Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 5)

6.7.1 Viewing Steps...............................................................................55
6.7.2 Viewing Real-Time Data With Profinia Software............................56
6.7.3 Clearing System Memory to Start the Run ....................................57
6.8 Monitoring Run Progress .........................................................................58
6.8.1 Run in Progress Screen................................................................58
6.8.2 Peak Detection .............................................................................59
6.8.3 Collecting the Purified Sample ......................................................61
6.8.4 Canceling or Pausing a Run .........................................................62
6.8.5 Completing the Run ......................................................................62
6.9 Preparing System for End-of-Run Cleaning .............................................62
6.9.1 Preparing for Line Cleaning ..........................................................62
6.9.2 System Cleaning in Progress........................................................63
6.10 Performing End-of-Run Procedures .........................................................63
6.10.1 Exporting Data to a Computer.......................................................64
6.10.2 Performing Final Run Procedures .................................................64
Section 7 Program Methods........................................................................68
7.1 Selecting Method System ........................................................................69
7.2 Selecting Method Type and Options ........................................................70
7.3 Entering Run and Sample Information .....................................................71
7.3.1 Adding or Editing Run and Sample Information.............................73
7.3.2 Adding Lot Number Tracking Information......................................73
7.4 Editing and Saving a Program Method.....................................................74
7.4.1 Editing a Program Method ............................................................74
7.4.2 Saving a Program Method ............................................................77
7.5 Installing Reagents, Sample Tubes, and Fraction Tubes .........................78
7.5.1 Preparing and Installing Reagents and Samples for the
Purification Run ............................................................................80
7.6 Installing Cartridges .................................................................................80
7.6.1 Installing the Sample Loop (Desalting-Only Methods) ...................81
7.7 Viewing Real-Time Data With Profinia Software.......................................83
7.8 Starting the Run.......................................................................................83
7.8.1 Viewing Steps...............................................................................84
7.8.2 Running the Manual Prime Function .............................................84
7.8.3 Clearing System Memory to Start Run ..........................................85
7.9 Monitoring Run Progress .........................................................................85
7.9.1 Peak Detection .............................................................................85
7.9.2 Collecting Purified Sample ............................................................86
7.9.3 Completing the Run ......................................................................86
7.10 Preparing System for End-of-Run Cleaning .............................................86
7.10.1 Preparing for Line Cleaning ..........................................................86
7.10.2 System Cleaning in Progress Screen............................................86
7.11 Performing End-of-Run Procedures .........................................................86
7.11.1 Exporting Data to a Computer.......................................................87
7.11.2 Performing Final Run Procedures .................................................87
Section 8 Saved Methods............................................................................89
8.1 Retrieving Saved Methods .......................................................................89
8.2 Selecting Saved Methods ........................................................................90
8.3 Running Saved Methods..........................................................................90
Section 9 Data and Utilities .........................................................................92
9.1 Exporting Data & Real-Time Data Acquisition ..........................................92
9.1.1 Exporting Data to USB Portable Memory Device ..........................92
ii