Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 135)

Fig. 11.5a. Installing the flow cell. Fig. 11.5b. Connecting the electrical connector.
Fig. 11.5c. Connecting the tubing. Fig. 11.5d. Installing the pH probe.
11.2 Larger Buffer, Sample, and Fraction Containers
The Profinia system is designed to operate with built-in components (buffer bottles and
fraction and sample tubes), but can accommodate larger bottles and tubes for large-sample
runs or program methods that require buffer volumes greater than those accommodated by
the Profinia 250 ml buffer bottle.
Important: Prior to starting a method, all the externally connected reagent tubes must be
primed manually. Use the manual priming function on the Start Run screen to do this.
11.2.1 External Buffer Containers
To connect an external buffer container to the Profinia instrument, all that is required is 1/8"
OD
37)(
tubing (catalog #750-0603 or similar), sufficient in length to connect the external
bottle(s) to the buffer inlet port on the instrument. To use buffer bottles that do not fit in the
system buffer bottle tray:
1. Place buffer bottle(s) to be used in your purification run next to the instrument.
2. Locate the bottom section of tubing that is connected to each buffer inlet valve.
3. Use one hand to push up on the silicone coupler, and the other to disconnect the short
piece of tubing by pulling it off.
4. Install the longer piece of 1/8" OD
37)(
tubing by pushing one end into the silicone
coupling (Figure 11.6).
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