Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 89)

Fig. 7.11. Start Run screen directs application of the sample to the sample loop (desalting-only method).
4. To apply sample, fill the syringe and insert it into the open female luer of the 3-way
stopcock. Turn the 3-way stopcock lever down toward the loop and fill the loop with
sample, avoiding bubbles as much as possible.
5. Return the 3-way stopcock lever to the middle position, facing toward the syringe. The
desalting loop is now filled with sample and ready for the desalting method to begin.
Fig. 7.12. Sample loop installed in the cartridge 1 position.
Note: Determine the appropriate combination of desalting loop and desalting cartridge for
the protein sample volume to be applied. For a 2 ml sample, choose the 2 ml desalting loop
and the 10 ml desalting cartridge. For a 10 ml sample, choose the 10 ml desalting loop and
the 50 ml desalting cartridge. Application of a sample volume smaller than the desalting
loop volume is not recommended, since it will result in increased dilution of the sample and
reduced desalting resolution.
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