Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 148)

Section 13
Care and Maintenance
Proper care and maintenance of Profinia ™ system parts help keep the instrument operating
at optimum performance levels.
13.1 Cleaning the Instrument
The Profinia instrument automatically flushes system lines at the end of each purification
run. To keep the system clean, there are several automated cleaning and storage procedures:
• Daily cleaning — buffer, sample, and fraction line cleaning
• Weekly cleaning — system NaOH and ethanol washes
• Storage
The procedures are selected from the Diag/Maint Functions menu, which is accessible via
the Data/Utilities menu. The procedures outlined below are designed to use water, ethanol,
or sodium hydroxide (NaOH). Other cleaning solutions, such as 0.5 M hydrochloric acid
(HCl), or 6 M guanidine HCl, may also be used. When using multiple cleaning solutions (for
example, 0.1 M NaOH followed by 0.5 M HCl), flush the system with water between steps
to prevent interactions between the cleaning chemicals.
Before running the cleaning procedures, remove the cartridges and connect the cartridge inlet
and outlet fittings by turning the plastic collars clockwise. Remove all fraction tubes and
place the cleaning tray in the fraction collection area. To run each cleaning procedure:
1. Touch the Data/Utilities button, then the Diag/Maint Functions button on the lower
toolbar to retrieve the menu for cleaning functions.
2. On the Diag/Maint Functions screen, touch the up and down arrows to scroll through
the list of functions and highlight the desired cleaning procedure.
3. Touch Select, then follow the touch screen instructions.
13.1.1 Daily Cleaning
When finished using the system for the day, perform buffer, sample, and fraction line
cleaning procedures as described in section 13.1.4.
13.1.2 Weekly Cleaning
Once a week, run the system NaOH wash, then the system ethanol wash as described in
section 13.1.4.
13.1.3 Storage
If the system will not be used for more than a week:
1. Run the system NaOH wash, then the system ethanol wash as described in section
13.1.4.
2. Empty the diluent and waste bottles.
3. Cap the ends of the buffer and sample inlet tubes and fraction outlet tubes with the
caps the instrument was shipped with (replacement Catalog #620-0444) to prevent the
entry of dust or foreign objects.
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