Bio-Rad Profinia™ Protein Purification Instrument User Manual

Page 32

Step 3: Cartridge Installation

If cartridges are not already installed, touch the
NO button. You will proceed to the Setup Cartridge
Positions screen.

If the cartridges required for the method are already
installed in the instrument, touch the YES. You will
proceed directly to the Start Run screen.

3A. Cartridge Line Priming

To connect cartridge holder fittings for priming:

1. Disconnect any cartridges.

2. Join the upper cartridge holder fittings directly to the

lower cartridge holder fittings in positions 1 and 2.

3. Touch the Start button on the Setup Cartridge

Positions screen to begin priming the cartridge lines.

■

Status is shown on the Priming In Progress screen

■

When priming is finished, the Install Cartridges
screen appears

3B. Install Cartridges

1. Disconnect the upper and lower cartridge

holder fittings.

2. Raise the upper cartridge holder fitting.

3. Remove the bottom cap from the affinity cartridge.

4. Position the cartridge in the lower cartridge

holder fitting and rotate the cartridge clockwise.
Tighten gently.

Upper
fitting

Lower
fitting

Upper and lower cartridge
holder fittings shown
directly connected and
ready for line priming.

Affinity cartridge
connected to lower
cartridge holder fitting.

1 Cartridge 2