Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 160)

Fig. 13.12. Align the flat side of the guides with the carrier assembly recess (tubing removed for clarity).
18. Use the 9/64" Allen wrench to tighten the screws. Start the inside screws first, alternating
between screws until they are finger-tight, then install the outside screws and tighten all
four.
19. Connect the 1/8" inlet tube to the pump head, and tighten the fitting with the fitting
wrench; do not overtighten the fitting.
20. Connect the 1/16" outlet tube to the pump head, and tighten the fitting with the fitting
wrench; do not overtighten the fitting.
21. Connect the 1/8" pump seal wash tubes to the top of the pump body.
22. Replace the access cover, reconnect the power cord and tubing, and turn the unit
around to face you.
23. Turn the instrument power on.
24. Place a bottle of 20% ethanol in buffer position 8.
25. Touch the Data/Utilities button on the system home screen.
26. Touch the Diag/Maint Functions button.
27. Touch the up and down arrows to select “System Ethanol Wash”, then press the Start
button. When the ethanol wash is complete, the system returns to the Diag/Maint
Functions screen.
28. Check the pump flow rate after changing piston seals (see section 9.2.2).
13.2.3 UV Monitor Lamp
When the UV lamp has passed its service life, it must be replaced. The most common
symptom of an expired lamp is a noisy UV baseline. Use the UV monitor utility outlined
below to check the lamp intensity.
Checking the UV Lamp Intensity
The UV monitor measures absorbance by comparing a reference voltage from a detector
that looks directly at the UV lamp, with a sample voltage from a detector that looks through
153
Flat side of
guides
Carrier
assembly
recess