Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 4)

Table of Contents
Page
Section 1 Profinia Protein Purification System Description .......................1
1.1 Profinia System Overview ..........................................................................1
1.2 Key Features of the Profinia System ..........................................................1
1.3 System Components and Optional Accessories.........................................2
1.4 Related Consumables and Products..........................................................4
1.5 About This Guide .......................................................................................4
1.5.1 Conventions....................................................................................4
Section 2 Installation Procedures.................................................................5
2.1 System Installation.....................................................................................5
2.2 First-Time Use of the Profinia System ........................................................8
2.2.1 Preparing the Instrument for Cleaning.............................................8
2.2.2 Selecting the First-Time Cleaning Procedure ..................................9
2.2.3 Setting the Local Date and Time ...................................................10
2.2.4 Validating the System ...................................................................10
Section 3 Key Terms and Definitions .........................................................14
3.1 Bio-Rad Methods .....................................................................................14
3.2 Program Methods ....................................................................................14
3.3 Toolbar Navigation Buttons......................................................................15
3.4 System Status Indicator Definitions..........................................................16
3.5 Alphanumeric and Numeric Keypads .......................................................17
3.5.1 Alphanumeric Keypad...................................................................17
3.5.2 Numeric Keypad ...........................................................................17
Section 4 Profinia System Quick Guide .....................................................19
4.1 Profinia Installation...................................................................................19
4.2 Profinia System Quick Guide ...................................................................23
Section 5 Home Screen Overview ..............................................................27
Section 6 Bio-Rad Methods.........................................................................29
6.1 Selecting Method System ........................................................................32
6.2 Selecting Method Type and Options ........................................................32
6.3 Selecting Sample Options........................................................................34
6.3.1 .........................Selecting Sample Flow Rate and Wash Time34
6.3.2 Selecting Sample Incubation Time................................................35
6.4 Entering Run and Sample Information .....................................................36
6.4.1 Adding or Editing Run and Sample Information.............................38
6.4.2 Adding Lot Number Tracking Information......................................38
6.5 Installing Reagents, Sample Tubes, and Fraction Tubes .........................39
6.5.1 Loading Buffers ............................................................................41
6.5.2 Installing Sample Tubes................................................................44
6.5.3 Installing Fraction Collection Tubes...............................................45
6.6 Installing Cartridges .................................................................................46
6.6.1 Preparing for Cartridge Installation................................................46
6.6.2 Setting Up Cartridge Positions for Priming ....................................47
6.6.3 Priming the Cartridge Lines...........................................................48
6.6.4 Installing Cartridges ......................................................................48
6.6.5 Installing the Sample Loop (Desalting-Only Methods) ...................52
6.7 Starting the Run.......................................................................................54
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