Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 143)

12.4.2 Chromatogram Overview
The following run parameters are displayed in the software chromatogram (Figure 12.3):
• UV-1 — data trace from the UV-1 detector
• Pressure — data trace from the pressure monitor
• pH — data trace from the pH monitor (optional accessory)
• UV-2 — data trace from the UV-2 detector
• Cond — data trace from the conductivity monitor
• Temp — data trace from the temperature monitor
• Main Steps — indicated by a downward-facing arrow symbol that marks the time at
which each main step occurred during the course of the run
• Fraction — indicated by “collection tube” symbol that marks the points at which
collection began during the course of the run
Fig. 12.3. Run parameters displayed at the top of a Profinia software chromatogram.
12.4.3 Eliminating Run Parameters Displayed
The top of each chromatogram displays all possible run parameters. Checked boxes indicate
the parameters displayed in the chromatogram. To remove any parameter from the
chromatogram, click on the box to remove the checkmark. The trace for that parameter as
well as any parameter-specific axis information will be removed from the screen.
12.4.4 Customizing Chromatogram Display Options
Customize the way information is displayed on your chromatogram by changing line colors
and widths, choosing whether UV-1 and UV-2 information is displayed on separate axes,
determining the major steps that are included in the chromatogram, and whether a sound is
heard at sample elution (real-time data display only).
Changing Chromatogram Background and Trace Colors
There are two categories of data for which you can customize the colors that are displayed:
• Chromatogram background — color of the entire chromatogram background
• Traces — color of each line that indicates UV, pressure, pH, conductivity, and fraction
information from the run
To change the colors that are used to distinguish information in your chromatogram:
1. Click “Options” > “Colors” from the software main menu.
2. Click on the bubble next to the parameter to change.
3. Click the Color button located at the bottom of the list of parameters.
4. Choose from Named or System Colors, or customize a color using the screen tabs.
Once a color has been selected, click the OK button to return to the Chromatogram
Option screen.
Note: Changes appear on the chromatogram as you make adjustments, but won’t be
saved until the entire procedure is completed.
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