beautypg.com

Bio-Rad Profinia™ Protein Purification Instrument User Manual

Page 209

background image

Appendix K
Profinia Program Methods Planning Worksheets

The worksheets included in this section are designed to help plan desired changes to step
parameters when customizing a program method. The worksheets provide an overview of
all main method steps and parameters associated with the steps; parameters that are not
editable are indicated by an “X” in the corresponding position. Worksheets are included for
the following method types:

Native IMAC only

Native IMAC plus desalting

Denaturing IMAC

GST only

GST plus desalting

Protein A and G only

Protein A and G plus desalting

Profinity eXact

Profinity eXact plus desalting

Affinity only

Affinity plus desalting

Desalting only

202