Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 136)

Fig. 11.6. Installing
37)(
tubing into the position 2 buffer line.
5. Place the other end of the tubing into the external buffer bottle.
Note: If connecting more than one external buffer bottle for a run, it is helpful to use stickers
to label each tube with the Profinia buffer position number marked on the buffer tray.
Once the longer tubes have been installed and other standard setup procedures are
complete, the system is ready for a purification run.
Note: The inlet tube must be primed before starting a run. This can be done using the
manual priming function in the method Start Run screen.
11.2.2 External Sample Containers
To connect an external sample container to the Profinia instrument, all that is required is
PTFE
tubing, 1/8" OD, sufficient in length to connect the external container(s) to the sample
inlet port(s) on the instrument. You need a length of tubing for each port that accommodates
the external sample containers. To use sample tubes that do not fit in the system sample
inlet tray:
1. Place external sample container(s) next to the instrument.
2. Locate the bottom section of tubing that is connected to each sample inlet valve.
3. Use one hand to push up on the silicone coupler, and the other to disconnect the tubing
by pulling it off.
4. Install the 1/8" OD
PTFE
tubing by pushing one end into the silicone coupling.
5. Place the other end of the tubing in the external sample container.
Note: If connecting more than one external sample container for a run, it is helpful to use
stickers to label each tube with the Profinia sample position number marked on the buffer
tray.
129