Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 150)

5. Place the cleaning tray in the fraction collection area.
6. Touch the Start button. A progress bar displays, tracking the duration of the procedure.
Cleaning Inlet and Outlet Lines
Use this procedure to clean all buffer and sample inlets and fraction outlets:
1. Fill diluent bottle with deionized water.
2. Fill seven buffer bottles with 50 ml of deionized water each, and place them in buffer
positions 1 through 7.
3. Fill one buffer bottle with 100 ml of 20% ethanol and place it in buffer position 8.
4. Fill two conical tubes with 25 ml of deionized water each, and place them in sample
positions 1 and 2.
5. Place the cleaning tray in the fraction collection area.
6. Touch the Start button. A progress bar displays, tracking the duration of the procedure.
Cleaning Pump Check Valves
Use this procedure to pump NaOH solution through the pump and clean the piston and
check valves:
1. Fill the diluent bottle with deionized water.
2. Fill one buffer bottle with 50 ml of NaOH solution (0.1 to 0.5 M) and place it in buffer
position 8.
3. Touch the Start button. A progress bar displays, tracking the duration of the procedure.
System NaOH Wash
Use this procedure to wash the system (except the buffer inlets) with NaOH solution:
1. Fill the diluent bottle with deionized water.
2. Fill one buffer bottle with 50 ml of NaOH solution (0.1 to 0.5 M) and place it in buffer
position 8.
3. Remove both cartridges from the system, and connect the inlet and outlet cartridge
fittings together.
4. Touch the Start button. A progress bar displays, tracking the duration of the procedure.
13.1.5 Cleaning Touch Screen
To clean the system touch screen:
1. Apply a neutral pH cleaner, such as ethanol or 3% or less isopropyl alcohol, to a soft,
lint-free cloth.
Note: Do not apply cleaning solution directly to the surface of the touch screen.
2. Wipe the touch screen gently with the cloth without allowing fluid or compressed air to
enter the small 1 mm vent hole along the edge of the touch screen.
Warning: Never use acidic or alkaline cleaners or organic chemicals, such as ammonia,
phosphates, ethylene glycol, paint thinner, acetone, toluene, xylene, propyl alcohol, or
kerosene to clean the Profinia touch screen.
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