Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 119)

9.3.11 Cleaning Inline Check Valve
Use this procedure to manually clean the inline check valve positioned between the
cartridge 2 outlet and the fraction valve (outlet selector valve); cleaning the inline check
valve manually with a syringe allows removal of residue as well as small particles:
1. Touch the Data/Utilities button on the system home screen.
2. Touch the Diag/Maint Functions button.
3. On the Diag/Maint Functions screen, touch the up and down arrows to scroll through
the list of functions and highlight “Clean Inline Check Valve”, then touch the Select
button.
4. Follow instructions displayed on the screen (Figure 9.15).
5. When the manual procedure is complete, touch the Finish button to wash the system
with water from the diluent bottle. A progress bar displays while cleaning is in process.
When cleaning is complete, the system returns to the Diag/Maint Functions screen.
Fig. 9.15. Clean Inline Check Valve screen.
9.3.12 Affinity Cartridge Cleaning in Place
A convenient cleaning in place method for prepacked 1 ml and 5 ml affinity chromatography
cartridges can be accessed through the Profinia Data/Utilities menu. This cleaning in place
method uses a wash buffer, a cleaning buffer, and a storage buffer. The formulation of
these buffers depends upon the specific type of affinity cartridge to be cleaned and the
nature of the contaminants to be removed. The instruction manual for prepacked affinity
chromatography cartridges contains pertinent information on compatible and recommended
cleaning reagents. Note that the formulation for the buffers should not be diluted and needs
to be supplied at the working concentration (1x).
1. Touch the Data/Utilities button on the system home screen.
2. Touch the Diag/Maint Functions button.
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