Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 144)

5. Repeat this procedure for each data category for which you’d like to implement a color
change. When finished, click Save to retain changes and return to the main software
screen, or click Close to return to the screen without implementing changes.
Fig. 12.4. Changing the color of the UV-1 trace line by selecting from the “Named” color list; it is also
possible to create custom colors.
Changing Trace Line Width
To change the thickness of trace lines:
1. Click “Options” > “Trace Options” from the software main menu.
2. Use the up and down arrows located in the “Width” box next to the parameter to
increase or decrease the width of its associated trace.
Note: Changes appear on the chromatogram as you make adjustments, but won’t be
saved until the entire procedure is completed.
3. Repeat this procedure for each data category for which you’d like to implement a trace
width change. When finished, click Save to retain changes and return to the main
software screen, or click Close to return to the screen without implementing changes.
Changing UV Axis Display
The software automatically generates separate axes for each UV detector. To merge UV
axis information into one y-axis:
1. Click “Options” from the software main menu.
2. Locate the “Separate Axis for UV-1 and UV-2 (Top)” and “Separate Axis for UV-1 and
UV-2 (Bottom)” menu options. A checkmark next to these menu options indicates the
function is in effect.
Note: Only two-sample runs have top and bottom chromatograms.
3. Click on the option for which you’d like to merge y-axis labels. The checkmark next to
the menu option disappears and UV-1 and UV-2 axis information displays together.
4. Repeat this procedure to separate UV-1 and UV-2 axis information.
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