Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 179)

Appendix C
Profinia
™ System Troubleshooting
Problems and Solutions
Problem Solution
No power to system If the instrument will not start, check that the system is
plugged in to a live outlet and that the power cord is inserted
into the rear panel receptacle. Make sure the switch is in the
on position. The Profinia system is compatible with 100–240
volt mains power without adjustments, and there are no
fuses or other user serviceable power protection devices
required.
Air in system The most common cause of air in the system is running out
of water or buffer. When this happens, the system will display
“Air in line detected!”. To solve this problem:
1. Examine all buffer and water containers and refill as
necessary.
2. Make sure all tubes are submerged in the liquid.
3. Touch the Start button. The system will purge the air, and
continue the separation run.
If all fluids are present, lift the instrument’s top cover to check
the inline filter fittings and the small pieces of tubing that
connect the sample and buffer inlet tubes. If necessary,
remove the fluidics compartment access cover and check for
loose fittings or damaged tubing.
System fails to detect The air detectors can be switched on and off manually. To
end of sample, or air make sure the end of sample/reagent detection feature is on:
in system
1. Touch the Data/Utilities button.
2. Touch the up and down arrows to scroll through the list of
functions and highlight “End of Sample/Regent
Detection”.
3. Select this function to make sure the air detectors are
switched on.
Clogged inline filter A clogged inline filter will reduce the system flow rate or
cause bubbles to appear in the flow stream. If the filter clogs
frequently, be sure to filter your sample with a 0.45 micron or
smaller membrane filter immediately before use. Use the
specified 10 micron membrane filter only; other filters may
restrict the flow or clog prematurely.
Fraction size small, Examine the sample tubes and verify that all of the sample
or flow rate low volume that was programmed has been injected into the
system.
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