Bio-Rad Profinia™ Protein Purification Instrument - Manual (Page 118)

9.3.10 Washing System With NaOH
Use this procedure to wash the internal system lines (including the cartridge lines) with
NaOH solution:
1. Touch the Data/Utilities button on the system home screen.
2. Touch the Diag/Maint Functions button.
3. On the Diag/Maint Functions screen, touch the up and down arrows to scroll through
the list of functions and highlight “System NaOH Wash”, then touch the Select button.
4. Follow instructions displayed on the screen (Figure 9.14).
5. Touch the Start button. The system slowly pumps NaOH through the system for 15 min,
followed by a system water wash at which point the system pauses. A progress bar
displays while cleaning is in process.
6. Follow screen instructions for the two available options: 1) Repeat the NaOH wash, or
2) Complete the wash (wash the buffer 8 position with water).
7. Touch the Repeat button to repeat the NaOH wash, or touch the Continue button to
complete the procedure. A progress bar will display while cleaning is in process.
When cleaning is complete, the system will return to the Diag/Maint Functions screen.
Fig. 9.14. System NaOH wash screen.
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