Metrohm 711 Liquino - Manual (Page 107)

4. Detailed description
711 Liquino, Instructions for use 101
Dosing units
If you only want to pipet without any dilutions then select a 2 mL or 5 mL
dosing unit. For dilutions a 10 mL or 20 mL dosing unit is recommended.
Parameters
The settings (pipetting volume, diluting volume, etc.) can be entered in the
parameter menu ( key) and stored as a method.
The
port allocation of the Dosino in the PIP mode is defined as follows:
4 2
1 3
Port allocation
Port 1
Dosing port
Port 2 Filling port (with dipper tube)
Port 3 empty
Port 4 Waste (for PREP function)
The port occupancy cannot be altered via
the keyboard.
Pipetting procedure (without pipet handle)
• Hold the pipet tip in a waste beaker and press the key. The
dosing cylinder and the tubing are rinsed and filled with solvent.
• Now press . If you have switched on the pipetting volume re-
quest in the parameter menu under 'required volume (up to 10 mL) and confirm with .
• The Dosino prepares for the aspiration process. The Liquino reports that
it is ready to aspirate the separating bubble.
• Remove the pipet tip from the waste vessel so that air can be aspirated.
Press the key.
• Air is now aspirated to form the separating bubble. The Liquino then re-
ports that it is ready to aspirate the sample.
• Immerse the pipet tip in the sample solution and press .
• The sample is now aspirated. The sample solution and the separating
bubble must now be completely contained in the pipet tubing. If sample
solution is drawn into the dosing unit you must use a longer pipetting
tubing.
• When the Liquino reports that it is ready to discharge the sample place
the pipet tip in the target vessel and press .
• The sample is now discharged. If a diluting volume has been entered
then the corresponding volume of solvent will also be automatically dis-
charged. The pipetting process is then complete.
• If no diluting volume has been selected then you will be requested to
carry out a rinsing procedure when the sample has been discharged.
Hold the pipet tip in a waste beaker and press . The separat-
ing bubble will then be discharged completely. The Liquino is then ready
for the next sample.