Metrohm 711 Liquino - Manual (Page 106)

4.7 PIP mode - precise pipetting and diluting
711 Liquino, Instructions for use 100
4.7 PIP mode - precise pipetting and diluting
The PIP mode of the 711 Liquino supports precise and comfortable pipet-
ting with the aid of a Dosino 700. In contrast to conventional dosing, in this
case the liquid to be pipetted is not aspirated into the dosing cylinder of the
Dosino, but only into the attached pipet tubing. In this way the liquid can be
aspirated rapidly and quickly and immediately discharged again. In order to
do this it is necessary to fill the dosing cylinder and tubing with a 'hydraulic
liquid'. A Dosino and dosing unit are mounted on a bottle containing the
same solvent as the solution to be pipetted.
Note
• Volumes from 50 µL to 10 mL can be pipetted.
• 50 mL dosing units cannot be used.
• The pipetted sample can be diluted with solvent in the same working
step.
• A separating bubble prevents mixing with the solvent.
• The sample to be pipetted must not be aspirated into the dosing cylin-
der.
• For samples in organic solvents the full accuracy is only guaranteed if
pipetting is followed by rinsing.
• Sample and target vessels (liquid levels) should have the same approx-
imate height to prevent hydrostatic effects from negatively influencing the
pipetting accuracy.
• It is absolutely necessary that the dosing unit is correctly prepared with
the PREP function before pipetting is carried out, i.e. tubing and dosing
cylinder are completely filled.
• The pipetting tip should be held at an angle of about 45° to the vessel
wall during pipetting - exactly the same position as is used with glass pi-
pets.
Pipetting equipment
With the aid of the optional pipetting equipment it is possible to pipet a
whole series completely controlled by the start button on the pipet handle.
The installation of the pipetting attachment is described on page 8 of this
‘Instructions for use’.
Pipet tip
Green pilot lamp
6.1562.040
Pipet handle
Black start
button
6.1562.050
Pipetting tubing
Connection to dosing
port 1 of Dosino 700 Connection cable to Liquino 711 (remote
connection)
Screw nipple