BINDER CB 60 - Manual (Page 19)

CB (E6) 09/2013 Page 19/144
2. Unit description
The CO
2
incubators CB are equipped with a multifunctional microprocessor display controller for
temperature, CO
2
, and O
2
(unit with O
2
control) levels and a digital display accurate to one-tenth of a
degree resp. 0.1 vol.-%
The inner chamber, the pre-heating chamber and the inside of the doors are all made of stainless steel
(material no. 1.4301 (V2A) in Germany). The inner surfaces are smooth and therefore easy to clean. The
inner chamber is deep-drawn from one piece, polished (suitable for pharmaceutical applications) and has
no welds or inaccessible corners. The hinges and the seal of the inner glass door are glued from the
outside to aid cleaning of the inner chamber. When operating the chamber at high temperatures
(sterilization), the impact of the oxygen in the air may cause discoloration of the metallic surfaces
(yellowish-brown or blue) by natural oxidation processes. These colorations are harmless and will in no
way impair the function or quality of the unit.
The perforated shelves are also made of stainless steel. You can insert a maximum of 3 (CB 60), 6 (CB
160), resp. 8 (CB 220) shelves.
The housing is RAL 7035 powder-coated. All corners and edges are also completely coated.
The heating system of the CO
2
incubator permits hot-air auto-sterilization at a setpoint of 187.5 °C /
369.5°F. Thus, a temperature of 180 °C / 356°F is maintained for at least 30 minutes on all internal
surfaces, resulting in sterilization of the entire inner chamber. Therefore, this procedure meets all
international guidelines regarding hot air sterilization, e.g. AAMI ST63, DIN 58947, European
Pharmacopoeia.
Thanks to the standard safety device (class 3.1 according to DIN 12880), the set temperature is
maintained in case of failure.
The gas enters the chamber via a fine filter (aseptic filter) with a high filtration efficiency that also filters
the smallest particles.
A highly precise, drift-free CO
2
infrared measuring system in combination with the permanent mixture of
CO
2
gas through a special proprietary gas mixing head developed by BINDER allows precise and
constant CO
2
concentrations for long periods. This creates optimum growth conditions for cultures.
The CO
2
incubator is also available with O
2
control in addition to CO
2
control.
CO
2
and O
2
sensors
Fast reaction times, maximum accuracy and selectivity characterize the CO
2
measuring procedure of
the CB incubator series. The accuracy of the CO
2
measuring system is based on a double-beam
infrared measuring cell with NDIR (non-dispersive infrared) sensor, which continuously regulates to a
reference value. Therefore, disturbance variables and aging phenomena in the measuring system are
almost completely eliminated, so that this measuring system, in contrast to other measuring
procedures, remains practically drift-free between calibrations and is entirely selective for CO
2
and
humidity. The sensor is built into the incubator and can be sterilized.
The O
2
sensor is a semiconductor gas sensor with ZrO
2
ceramic.
The accuracy of the indicated values of CO
2
and O
2
(unit with O
2
control) depends on the ambient air
pressure (approx. 0.08 vol.-% per 10 mbar / 0.15 psi). To compensate for this effect in the CO
2
measurement, the controller measures the ambient air pressure and automatically includes it in the
calculation.
The CO
2
incubators CB are equipped with an Ethernet interface for computer communication, e.g. with
the communication software APT-COM™ 3 DataControlSystem (option, chap. 15.1). For further options,
see chap. 22.5.
Temperature range: 7 °C / 12.6 °F above ambient temperature up to +60 °C / 140 °F
CO
2
range: 0 vol.-% up to 20 vol.-%)
O
2
range (unit with O
2
control): 0.2 vol.-% up to 95 vol.-%