BINDER CB 60 - Manual (Page 104)

CB (E6) 09/2013 Page 104/144
• Reduce the number of personnel and their movements in the lab by careful positioning all relevant
equipment. For practical reasons, install the CO
2
incubator close to the laminar air flow bench.
• Regular microbiological monitoring of the cell culture laboratory.
17.3 Working and behavior in the lab
Sources of contamination are often the laboratory personnel themselves (surface germs, oral flora droplet
transfer) and handling the equipment and cultures. We recommend staff training in aseptic techniques,
laboratory safety and good laboratory practice (GLP).
Examples of general rules to reduce the contamination risks
• Reduce hand germ count (wash hands with antimicrobial soap, dry with paper tissues, and rub dry
hands with alcohol-based solution).
• Wear appropriate clothing (work coat, shoes, face mask)
• Keep as few personnel as possible in the cell culture lab.
Examples of sterile working method
• Work "clean-to-dirty", i.e., handle confirmed uncontaminated cells first, unknown or untested cells next,
and lastly, if necessary, cells suspected to be contaminated.
• Perform daily microscopic observations of cultures and specific tests for the bacteria and fungi as part
of a controlled routine. Test cultures for sterility before starting work.
• Keep working surfaces clean. Immediately wipe spilled liquids with alcohol solutions.
• No mouth contact on pipettes.
• Never work on top of open sterile containers.
17.4 Chamber design and equipment of the CO
2
incubator
The design concepts behind the CO
2
incubator CB considerably reduce the risk of contamination. Among
them are:
Even surfaces for easy manual cleaning
• The inner surfaces are smooth and therefore easy to clean. The inner chamber is deep-drawn from a
single piece, polished (suitable for pharmaceutical work) and has no welds or inaccessible corners.
The hinges and the seal of the inner glass door are glued from the outside, which also aids cleaning of
the inner chamber.
Removable parts for cleaning and autoclaving
• The shelves are easily removed without screws. It is possible to autoclave the shelves. But this is
generally not necessary because they can remain inside the incubator during sterilization.
Door gasket
• The inner door gasket is removable and autoclavable.
Gas fine filter
• The incoming gas used in the operation passes through a fine filter (aseptic filter, filtration efficiency
99.99 %, particle size 0.45 µm) with a high filtration efficiency, which can also filter the smallest
particles.
CO
2
measuring system in the inner chamber
• The CO
2
sensor can be hot-air sterilized inside the chamber.
O
2
measuring system in the inner chamber
• The O
2
sensor can be hot-air sterilized inside the chamber.