BINDER CB 60 - Manual (Page 18)

CB (E6) 09/2013 Page 18/144
1.7 Precautions when handling gas cylinders
General information for safe handling of gas cylinders:
•
Store and use gas cylinders only in well-ventilated locations.
• Open the gas cylinder valve slowly to avoid pressure surges.
• Secure gas cylinders during storage and use against falling (chaining).
• Transport gas cylinders with a cylinder cart, do not carry, roll, or throw them.
• Always close the valve even with apparently empty cylinders; screw on the cap when not in
use. Return gas cylinders with the valve closed.
• Do not open gas cylinders by force. Mark them when damaged.
• Protect gas cylinders against fire, e.g. do not store together with flammable liquids.
• Observe relevant regulations for dealing with gas cylinders.
Secure the gas cylinders against falling and other mechanical damage.
WARNING
Safety valve tearing off.
Sudden release of the stored pressure energy.
Risk of injury.

Secure gas cylinders against falling (chaining).
 Transport gas cylinders with a cylinder cart.
The valve of the gas cylinder always must be closed before screwing on or unscrewing the gas hose.
WARNING
Opening the cylinder valve when the cylinder is not connected.
Sudden release of the stored pressure energy.
Risk of injury.

Close the gas cylinder valve before connecting or removing the gas hose.
After connecting the gas cylinder, check all gas connections for leaks (e.g. with leak spray or
diluted soap solution).
1.8 Intended use
Series CB incubators are suitable for the cultivation of mammal cells under typical conditions of approx.
37 °C / 98.6°F. The incubator permits setting defined pH conditions by common NaHCO
3
buffer systems
of commercial cell media by keeping an exact CO
2
atmosphere inside. CB incubators guarantee high
humidity inside to avoid osmolarity increasing caused by the evaporation of the cell media.
With the unit with O
2
control, a variable oxygen atmosphere can additionally influence the growth of the
cells.
Following the instructions in this operating manual and conducting regular maintenance work
(chap. 19.1) are part of the intended use.
Other applications are not approved.
WARNING: If customer should use a BINDER chamber running in non-supervised
continuous operation, we strongly recommend in case of inclusion of irrecoverable specimen
or samples to split such specimen or samples and store them in at least two chambers, if this
is feasible.