Luminex 100 IS Version 2.2 - Manual (Page 9)

x MAP Technology General Guidelines
PN 89-00002-00-032 Rev. A 3
• Floppy Disk: 1.4 MB 3 1/2 inch
• 100 MB read/write CD ROM
• Analyze multiple 96-well plates per batch
• Analyze multiple assay templates per plate
• Distinguish a minimum of 1 to a maximum of 100 unique
x MAP microsphere sets in a single sample
• Detect and distinguish reporter fluorescence emissions at 575 nm
on the surface of 1 to 100 unique
x MAP microsphere sets in a
single sample
• Sample uptake volumes from 20 to 200 µL
• Sample core: 15-20 µm core at 1 µL/sec. sample injection rate
• Sample timeout limit from 1 to 400 seconds
• Maintain samples at a constant temperature from 35°C to 55°C
(95°F to 131°F)
• Automatic sampling from a 96-well plate
• Start sampling from any well position
• Sheath container and waste container hold enough volume to run
up to two 96-well plates between refills
• Microtiter Plates with 96-wells must be compatible with the
Luminex XY Platform (XYP
™ ) plate holder. The following plate
holder types are compatible with the XYP plate holder: flat
bottom, conical, round, filter bottom, and half-well plates with
overall height no more than 0.75 inches (19 mm), any color.
• Microtiter plates with 96-wells must be compatible with XYP
heater block temperatures from 35°C to 55°C (95°F to 131°F)
General Guidelines
Bead Handling
Limitations Follow these instructions to ensure consistency when using the
microspheres. Microspheres are susceptible to photobleaching, and
photobleaching effects are cumulative. Minimize exposure of
microspheres to light during your development and manufacturing
phases to maintain product integrity. The microspheres are
hydrophobic in the aqueous medium provided. The microspheres
will settle if left undisturbed and require resuspension prior to
dispensing. Do not use this product with strong organic solvents (call
Luminex Customer Support for specific compatibility).