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Luminex 100 IS Version 2.2 User Manual

Page 89

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PN 89-00002-00-032 Rev. A

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MAP

Technology

3. The optimal coupling concentration for a given oligonucleotide

is determined by coupling at various concentrations within the
recommended range of 1 to 50 µM.

4. Minimize the exposure of EDC to air; secure closures on stock

and dry aliquot containers, store desiccated at -20°C. Use
aliquots immediately and discard containers after use. Make a
fresh 10 mg/mL EDC solution before each addition.