Luminex 100 IS Version 2.2 - Manual (Page 20)

Luminex 100 IS Developer Guide to x MAP Technology Version 2.2 x MAP Technology
14 PN 89-00002-00-032 Rev. A
When you replay a batch it labels unknown samples as Pa1, Pa2,
Pa3, and so on.
If you replay a batch containing replicates, replicate averaging will
not be calculated in data analysis.
A number of variables can affect the final test results. You may also
change the standards or controls processed with the batch or multi-
batch. These variables may effect your test results:
• minimum number of events for acquisition
• formula used to analyze the MFI values
• standards or controls validation or invalidation
• type of analysis (qualitative, quantitative, acquisition only, or
maintenance)
Reprocess
Samples Using
Replay Batch
You reprocess samples using Replay Batch from the Acquisition
Detail tab.
‹ To reprocess samples using Replay Batch:
1. Click the Acquisition Detail tab.
2. Click Replay Batch on the Acquisition Detail tab. A Browse for
Folder dialog box appears displaying the My Batches directory
(folder).
3. Select the desired batch under the My Batches folder and click
OK.
4. The Open Template dialog box appears. Select the desired
template and click Select.
5. The Run Batch tab becomes the active tab. You can monitor the
commands as they process. Switch to the Acquisition Detail tab
and monitor the data, histogram, and dot plot.
Analyze
Reprocessed Data
with Replay Batch
After running Replay Batch, you can analyze the data.
To analyze Replay Batch data:
1. Select Start Analysis on the toolbar.
2. At the Open Batch dialog box select the batch you want to
analyze and click Select. The most recent Replay Batch is the
last or has the highest ID number. See Figure 5.