Luminex 100 IS Version 2.2 - Manual (Page 10)

Luminex 100 IS Developer Guide to x MAP Technology Version 2.2 x MAP Technology
4 PN 89-00002-00-032 Rev. A
Safety Precautions Although this product is not known to contain hazardous or
carcinogenic components at toxic levels, it may be toxic if inhaled, if
it comes in contact with skin, or if swallowed. There may be danger
of cumulative effects. Keep away from food, drink, and animal food
items. If product comes in contact with skin, wash immediately with
plenty of water. Wear suitable protective clothing. In case of accident
or if you feel unwell, seek medical advice immediately and show this
product label or container to your medical provider. Material Safety
Data Sheet available upon request.
Warning: The Luminex 100 IS instrument contains dangerous
lasers. Do not open the instrument’s cover. Removing the instrument
cover voids the warranty. Be sure to read the Safety Chapter in the
“Luminex 100 IS User Manual Version 2.2” before proceeding.
Handling
Instructions
Luminex x MAP Carboxylated Microspheres are provided in various
sized containers. The handling of the product varies with the size of
container. Take care to avoid excessive agitation of the product and
resultant foaming/surface precipitation. The microspheres will settle
if left undisturbed. Always ensure that microspheres are
homogeneously resuspended prior to dispensing or analyzing. The
uncoupled microspheres are not monodispersed and tend to
aggregate until coated. Multiple pipetting from the original container
may affect bead concentrations. Protect the microspheres from light
at all times. Follow the resuspension instructions based on the size of
container in use:
For 1 mL containers: Remove from 2-8°C and allow equilibration to
room temperature. Centrifuge for 1-2 minutes at 10,000 x g. Sonicate
in a water bath sonicator to disperse the microsphere pellet. Mix by
gentle vortexing immediately prior to dispensing. Inversion is not
recommended.
For larger containers: Remove from 2-8°C and allow equilibration to
room temperature. Place on rotator and rotate for a minimum of 15
minutes. Mix by gentle inversion immediately prior to dispensing.
For these containers, vortexing and rocking are not recommended.
Bead Selection
You select beads from the standard 100-region bead map. Refer to
Table 1. Luminex recommends that you select beads starting from
the upper left, reading across to the end of a row, and then down to
the next row: