beautypg.com

Performance specifications, Speed, Accuracy and precision – Luminex 100 IS Version 2.2 User Manual

Page 8: Sensitivity, Capacity

background image

Luminex

100

IS Developer Guide to

x

MAP Technology Version 2.2

x

MAP

Technology

2

PN 89-00002-00-032 Rev. A

Performance

Specifications

Speed

933 MHz Intel

®

Pentium

®

III processor or faster

Memory: 256 MB RAM

USB communications link for fast data transfer

Automatic transfer of assay templates and new reagent
information into the system using a 3 1/2 inch floppy disk or
large capacity read/write CD

Luminex 100 IS system installation < 4 hours

System calibration < 10 minutes

System controls < 10 minutes

Barcode reader entry of sample IDs

Automatic post analysis

Analyze one 96-well plate/hour depending on manufacturer’s kit

Up to 100

x

MAP microsphere sets per sample

Sheath flowrate 90

µL/sec. ± 5 µL

Sample injection rate into detector area 1

µL/sec. ± 0.05 µL

System warmup 30 minutes. Systems that remain inactive for at
least four hours will require a warm-up to restart the lasers. After
acquiring sample, running system calibrators, running system
controls, and warming up the instrument, the system resets the
four-hour internal clock.

Accuracy and Precision

Sample uptake volume accuracy ± 5%

Classification of

x

MAP microspheres > 80.0%

Misclassification of

x

MAP microspheres < 0.5%

Temperature control 0°C to ± 3°C of target

Internal sample carry over < 0.9%

Soluble background fluorescence emission at 575 nm
automatically subtracted from median fluorescence intensity
value

Sensitivity

Detect at least 1000 Phycoerythrin fluorochromes per
microsphere

Reporter channel dynamic range: 3.5 decades

Capacity

Minimum values:

Hard Drive: 10 GB

Store data for up to 240,000 test results