Performance specifications, Speed, Accuracy and precision – Luminex 100 IS Version 2.2 User Manual
Page 8: Sensitivity, Capacity

Luminex
100
IS Developer Guide to
x
MAP Technology Version 2.2
x
MAP
Technology
2
PN 89-00002-00-032 Rev. A
Performance
Specifications
Speed
•
933 MHz Intel
®
Pentium
®
III processor or faster
•
Memory: 256 MB RAM
•
USB communications link for fast data transfer
•
Automatic transfer of assay templates and new reagent
information into the system using a 3 1/2 inch floppy disk or
large capacity read/write CD
•
Luminex 100 IS system installation < 4 hours
•
System calibration < 10 minutes
•
System controls < 10 minutes
•
Barcode reader entry of sample IDs
•
Automatic post analysis
•
Analyze one 96-well plate/hour depending on manufacturer’s kit
•
Up to 100
x
MAP microsphere sets per sample
•
Sheath flowrate 90
µL/sec. ± 5 µL
•
Sample injection rate into detector area 1
µL/sec. ± 0.05 µL
•
System warmup 30 minutes. Systems that remain inactive for at
least four hours will require a warm-up to restart the lasers. After
acquiring sample, running system calibrators, running system
controls, and warming up the instrument, the system resets the
four-hour internal clock.
Accuracy and Precision
•
Sample uptake volume accuracy ± 5%
•
Classification of
x
MAP microspheres > 80.0%
•
Misclassification of
x
MAP microspheres < 0.5%
•
Temperature control 0°C to ± 3°C of target
•
Internal sample carry over < 0.9%
•
Soluble background fluorescence emission at 575 nm
automatically subtracted from median fluorescence intensity
value
Sensitivity
•
Detect at least 1000 Phycoerythrin fluorochromes per
microsphere
•
Reporter channel dynamic range: 3.5 decades
Capacity
Minimum values:
•
Hard Drive: 10 GB
•
Store data for up to 240,000 test results