Luminex 100 IS Version 2.2 - Manual (Page 81)

PN 89-00002-00-032 Rev. A 75
x
MAP
Protocols
Updates or additions to these protocols are posted on the Luminex
website at
http://www.luminexcorp.com/ats .
The protocols in this appendix are presented in the following order:
‰ Two-Step Carbodiimide Coupling of Protein to Luminex
Carboxylated Microspheres
‰ One-Step Carbodiimide Coupling of Oligonucleotides to
Luminex Carboxylated Microspheres
‰ Binding Biotin-conjugated Molecules to LumAvidin
®
-Modified
Microspheres
Microsphere Handling
Microsphere dispersion
Luminex x MAP microspheres settle and aggregate if left
undisturbed. You should always ensure that they are evenly
distributed before dispensing. Gentle vortexing is an effective
method of mixing for most coated microsphere preparations. After
dispersion, microspheres generally remain in solution for about one
hour. To maintain bead concentration within the stock microsphere
volumes (1 mL, 4 mL, or 16 mL), we suggest that you follow these
guidelines.
For complete resuspension of the 1 mL stock vials, Luminex
recommends centrifuging, sonicating, and vortexing. Failure to do so
often results in decreased amounts of microspheres recovered in
future sample aliquots.
Larger 4 mL and 16 mL vials should be placed on a rotator for a
minimum of 15 minutes for complete resuspension. Vortexing and
sonicating are not recommended for the larger vials as they are not
B