BUCHI PrepChrom C-700 - Manual (Page 78)

6 | Operation BÜCHI Labortechnik AG
78/110 PrepChrom C­700
• Step gradient mode
Note
The first step is used as a start point only and corresponds to the condition used
during equilibration (if activated). The time of the first step can not be modified.
u Adjust the flow rate (1).
Note
When using the BUCHI column methods, the system sets the optimal flow rate
for the column (only flash columns).
u Adjust the percentage of the used solvents (2).
Note
Always start adjusting the solvent percentages from the right to the left.
u Add the second step (3).
u Set the end time for the second step (4).
Note
The flow rate and the percentage of the used solvents in the second step are
equal to the first step (= equilibration step) and can not be modified separately.
u Add other steps for elution (3) and adjust the flow rate and percentage of
the solvents.
u Select the tab Detection.