BUCHI PrepChrom C-700 User Manual
Page 77

BÜCHI Labortechnik AG
Operation | 6
PrepChrom C700
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Select the tab Elution.
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Press the icon (1) to switch between the graphical and classical table.
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Press the gradient mode button (2) to choose between linear and step gra
dient mode.
• Linear gradient mode
Note
The first step is used as a start point only and corresponds to the condition used
during equilibration (if activated). The time of the first step can not be modified.
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Adjust the flow rate (1)
Note
When using BUCHI column methods, the system sets the optimal flow rate for
the column (only flash columns).
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Adjust the percentage of the used solvents (2).
Note
Always start adjusting the solvent percentages from the right to the left.
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Add other steps (3).
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Set the end time for elution (4).