Ocean Optics SpectraSuite - Manual (Page 94)

7: Processing Menu Functions
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Pre-processing
Preprocessing functions currently include the following:
 Nonunity correction
 Add Reference Monitor
You can also remove the preprocessing function.
Remove Preprocessor
Select Processing | Pre-processing | Remove Preprocessor to remove the nonunity correction
preprocessing function.
Nonunity Correction
Select Processing | Pre-processing | Non-unity correction for reflection experiments if you need to
perform a calculation to compensate for a reflection standard that is not perfectly white. You can also
access this function with the icon in the Preprocessing toolbar.
SpectraSuite provides the icon for storing a reference. The default assumption is that the reference is
equal to exactly 100% reflection. This works for comparative work, but it will not yield accurate
quantitative results.
The better procedure is to use a file of nominal reflectivity values or NIST-traceable values for your
particular standard. These files are available for download from this page for the WS-1-SL, STAN-SSH
and STAN-SSL standards. If you are using a WS-1, there are significant potential errors. The WS-1-SL is
recommended as a substitute.
► Procedure
1. Set up your experiment as usual by choosing the appropriate integration period (so the signal to
noise will be high but the spectra for the standard or samples won't be off-scale). Choose scans to
average and box car smoothing to obtain the desired signal-to-noise.
2. Store dark and reference spectra and switch to %R (or %T) scales. SpectraSuite will make your
graph read 100% +/- random noise across the spectra.
3. Click on the icon.