Ocean Optics SpectraSuite - Manual (Page 160)

A: Experiment Tutorials
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Typical transmission setup: The light source (far right) sends light via the input leg of a transmission
probe into a container (bottom center). The light interacts with the sample. The output leg of the
transmission probe carries the information to the spectrometer (top center), which transmits the
information to the computer (far left).
Common transmission applications include measuring light through solutions, optical filters, optical
coatings, and other optical elements (such as lenses and fibers).
► Procedure
Perform the following steps to take a transmission measurement using SpectraSuite:
1. Place SpectraSuite in Scope mode by clicking the Scope ( ) icon in the Experiment mode
toolbar or selecting Processing | Processing Mode | Scope from the menu.
2. Ensure that the entire signal is on scale. The intensity of the reference signal’s peak differs
depending on the device being used. If necessary, adjust the integration time until the intensity is
appropriate for your device.
3. If you have not already done so, place a sample of the solvent into a cuvette and take a reference
spectrum. You must take a reference spectrum before measuring transmission.