Ocean Optics SpectraSuite - Manual (Page 149)

A: Experiment Tutorials
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8. Block the light path to the spectrometer, uncheck the Strobe/Lamp Enable box, or turn the light
source off. Then, take a dark spectrum by clicking . You must take a dark spectrum before
measuring absorbance.
This command merely stores a dark spectrum in memory. You must click the Save Spectra ( )
icon on the toolbar to permanently save the spectrum to disk.
Note
If possible, do not turn off the light source when taking a dark spectrum. If you must turn
off your light source to store a dark spectrum, allow enough time for the lamp to warm up
again before continuing your experiment. After the lamp warms up again, store a new
reference.
9. Put the sample in place and ensure that the light path is clear. Then, click .
If you have already taken one or more absorbance measurements, a dialog box appears asking
you to specify whether to display the new data in a new graph, or on the existing graph.