Ocean Optics SpectraSuite - Manual (Page 150)

A: Experiment Tutorials
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Note the following changes on the screen:
 The experiment mode listed in the Data Sources and Data Views panes changes to
Absorbance Mode.
 The units listed on the Graph pane changes to Absorbance (OD).
10. To permanently save the spectrum to disk, click the Save Spectra ( ) icon on the toolbar.
Note
If you change any sampling variable (integration time, averaging, smoothing, fiber size,
etc.), you must store a new reference and dark spectrum.
Concentration Experiments
To discover the unknown concentration of a substance in a solution, you must first take spectral scans of a
series of solutions with different known concentrations of the same substance. You begin this process by
taking an absorbance spectrum of the solution with the highest known concentration.
While going through the wizard, when asked to store a reference spectrum, put a cuvette full of your
solvent in the spectrometer’s cuvette holder.
There are three methods you can use to calculate the concentration of a sample:
 Using Beer’s Law (see Calculating Concentration Using Beer’s Law )
 Calibrating from a solution of known concentration (see Calibrating from Solutions of Known
Concentration )
 Loading a previously stored calibration from a file (see Loading Calibration from an Existing File )
The Concentration Measurement Setup Wizard allows you to choose one of these three methods.