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New absolute irradiance measurement, New concentration measurement – Ocean Optics SpectraSuite User Manual

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4: File Menu Functions

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New Absolute Irradiance Measurement

The New Absolute Irradiance Measurement menu selection launches the Absolute Irradiance

Wizard to facilitate taking an absolute irradiance measurement using SpectraSuite. The wizard leads you
through the steps necessary to take an absolute irradiance measurement:

1. Selecting your source

2. Setting the data acquisition parameters

3. Taking a reference spectrum

4. Taking a dark spectrum

5. Calibrating the spectrometer

See

Absolute Irradiance

for more information on taking an absolute irradiance measurement.

New Concentration Measurement

Concentration is the amount of a specified substance in a solution. Graphs of absorbance vs.

concentration are known as Beer’s Law plots. These are prepared by measuring the light absorbed by a
series of solutions with different known concentrations. The length of the sample, such as the path length
of our cuvette holder, and the wavelength chosen for monitoring the amount of light absorbed are
constants. A linear plot from taking scans of these standard solutions with known concentrations is then
obtained. The plot is then used to determine the unknown concentrations of substances in solutions.