Ocean Optics SpectraSuite - Manual (Page 178)

A: Experiment Tutorials
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5. If you have not already done so, store a dark spectrum reading. To do this, either block the light
path to the spectrometer, uncheck the Strobe/Lamp Enable box in the Acquisition toolbar, or
turn the light source off. Then, take a dark spectrum by clicking the Store Dark Spectrum (
)
icon on the toolbar or by selecting File | Store | Store Dark Spectrum from the menu bar. This
command merely stores a dark spectrum in memory. You must click the Save Spectra ( ) icon
on the toolbar.
Note
If possible, do not turn off the light source when taking a dark spectrum. If you must turn
off your light source to store a dark spectrum, allow enough time for the lamp to warm up
again before continuing your experiment.
You must take a dark spectrum before measuring relative irradiance.
6. Position the fiber at the light source you want to measure. Then, click . The Relative
Irradiance graph appears.