Ocean Optics SpectraSuite - Manual (Page 152)

A: Experiment Tutorials
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Calculating Concentration Using Beer’s Law
a. Select Beer-Lambert Law to specify the molar extinction constant  and a wavelength. Then,
Click .
The following window appears:
b. Fill in the fields according to the constants appropriate for your sample. The Compound
Name and Concentration Units can be set to anything, and are simply provided for
reference. Click when done.
Calibrating from Solutions of Known Concentration
a. Insert a sample for which the concentration is known into the spectrometer.
b. Select Calibrate from solutions of known concentration, insert samples into the
spectrometer for which you know the concentration and use that as a basis for computing the
concentration of the unknowns. Then, click . The following window appears: