Ocean Optics Ocean View Install - Manual (Page 60)

4: Wizards
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Beer's Law
5. Use the Beer's Law panel to set the parameters for your Beer-Lambert concentration
experiment.
a. The Wavelength box determines at what wavelength the absorbance will be
evaluated. The concentration based on absorbance at this wavelength will be displayed in
a scalar window upon completion of the wizard.
b. Concentration Units allows you to enter in the appropriate units for display in the scalar
window. Note that the units for the Molar Extinction Coefficient units will update when
you click off of this box.
c. Molar Extinction allows you to enter the Molar Extinction Coefficient of your
solution. Note that the units on this coefficient are set by the previous box. For your
final concentration to be displayed correctly, the Molar Extinction Coefficient must be
converted to the appropriate units.
d. Path Length allows you to enter the path length of the sample holder used in your
concentration experiment. Note that the units of path length default to centimeters and
cannot be changed.
e. Compound Name allows you to set the name of the solution used in your concentration
experiment. This name will be displayed in the scalar window upon completion of the
wizard.
6. Click Finish to complete and exit the wizard.
--OR--
Known Solutions
5. Use Calibrate from solutions of known concentration to calibrate your concentration
experiment using solutions of known concentration.
- If you have a valid concentration file from a previous experiment that you would like to use,
click Load File and select the appropriate .con file. The file will populate all necessary fields.
- --OR--
- If you are beginning a new experiment, begin by entering the Compound Name and
Concentration Units in the boxes at the top of the panel. The Notes box is convenient for
capturing any comments pertaining to the experiment.
a. Build a table of known concentration and absorbance values. Begin by placing a sample
of known concentration in the sample holder.
b. Enter the sample’s concentration in the Concentration box.
c. Click Get Absorbance. Note that you may need to click Get Absorbance twice to get
an accurate value.
d. When you are satisfied with the absorbance value for the given concentration, click Add
Sample Point to add the values to the data table.
e. Remove the sample and place a sample with a different concentration in the sample
holder.
f. Repeat the above steps C -- E for all of your known sample concentrations.